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mirna labeling, quality control, chip hybridization, and microarray analysis  (CapitalBio Corporation)

 
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    CapitalBio Corporation mirna labeling, quality control, chip hybridization, and microarray analysis
    Mirna Labeling, Quality Control, Chip Hybridization, And Microarray Analysis, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+microarray+hybridization/mirna+labeling++quality+control++chip+hybridization+and+microarray+analysis/pm28487996-76-16-4
    Average 90 stars, based on 1 article reviews
    mirna labeling, quality control, chip hybridization, and microarray analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system
    Article Snippet: Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. RNA isolation and qRT-PCR of mature miRNAs Total RNA was extracted from cells or tissues using Trizol reagent (Invitrogen). qRT-PCR was carried out using TaqMan miRNA probes (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

    Article Title: Identification of miRNAs that are associated with tumor metastasis in neuroblastoma.
    Article Snippet: 39 .6 9] a t 0 4: 12 3 0 A ug us t 2 01 5 450 Cancer Biology & Therapy Volume 9 Issue 6 previously described.35,36 Briefly, 50 μg total RNA was purified using mirVANA miRNA isolation kit (Ambion, Inc.) to enrich small RNA fraction. .. Purified RNA was labeled with fluorescein and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human and mouse miRNA genes. ..

    Article Title: δ-Opioid Receptor Activation Modified MicroRNA Expression in the Rat Kidney under Prolonged Hypoxia
    Article Snippet: In brief, 50 μg of total RNA were purified using the mirVANA miRNA isolation kit (Ambion, Carlsbad, CA, USA) to enrich for the small RNA fraction. .. The purified RNA was labeled with Cy3 and hybridization was carried out on a miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: Role of miR-143 targeting KRAS in colorectal tumorigenesis.
    Article Snippet: Briefly, 50mg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction. .. Purified RNA was labeled with Cy3 and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Labeling:

    Article Title: Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system
    Article Snippet: Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. RNA isolation and qRT-PCR of mature miRNAs Total RNA was extracted from cells or tissues using Trizol reagent (Invitrogen). qRT-PCR was carried out using TaqMan miRNA probes (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

    Article Title: Identification of miRNAs that are associated with tumor metastasis in neuroblastoma.
    Article Snippet: 39 .6 9] a t 0 4: 12 3 0 A ug us t 2 01 5 450 Cancer Biology & Therapy Volume 9 Issue 6 previously described.35,36 Briefly, 50 μg total RNA was purified using mirVANA miRNA isolation kit (Ambion, Inc.) to enrich small RNA fraction. .. Purified RNA was labeled with fluorescein and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human and mouse miRNA genes. ..

    Article Title: δ-Opioid Receptor Activation Modified MicroRNA Expression in the Rat Kidney under Prolonged Hypoxia
    Article Snippet: In brief, 50 μg of total RNA were purified using the mirVANA miRNA isolation kit (Ambion, Carlsbad, CA, USA) to enrich for the small RNA fraction. .. The purified RNA was labeled with Cy3 and hybridization was carried out on a miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: Role of miR-143 targeting KRAS in colorectal tumorigenesis.
    Article Snippet: Briefly, 50mg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction. .. Purified RNA was labeled with Cy3 and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Hybridization:

    Article Title: Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system
    Article Snippet: Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. RNA isolation and qRT-PCR of mature miRNAs Total RNA was extracted from cells or tissues using Trizol reagent (Invitrogen). qRT-PCR was carried out using TaqMan miRNA probes (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

    Article Title: Identification of miRNAs that are associated with tumor metastasis in neuroblastoma.
    Article Snippet: 39 .6 9] a t 0 4: 12 3 0 A ug us t 2 01 5 450 Cancer Biology & Therapy Volume 9 Issue 6 previously described.35,36 Briefly, 50 μg total RNA was purified using mirVANA miRNA isolation kit (Ambion, Inc.) to enrich small RNA fraction. .. Purified RNA was labeled with fluorescein and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human and mouse miRNA genes. ..

    Article Title: δ-Opioid Receptor Activation Modified MicroRNA Expression in the Rat Kidney under Prolonged Hypoxia
    Article Snippet: In brief, 50 μg of total RNA were purified using the mirVANA miRNA isolation kit (Ambion, Carlsbad, CA, USA) to enrich for the small RNA fraction. .. The purified RNA was labeled with Cy3 and hybridization was carried out on a miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: Ethanol exposure induces differential microRNA and target gene expression and teratogenic effects which can be suppressed by folic acid supplementation.
    Article Snippet: Briefly, total RNA was extracted from E17.5 fetus brains using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. An aliquot (5 mg) of the RNA sample was used for hybridization on miRNA microarray chips (CapitalBio Co., Beijing, China) which contained 509 mature miRNA probes spotted in quadruplicate (435 for human, 261 for mouse and 196 for rat) with annotated active sites, according to the miRNA database at Sanger Center in October 2005 (http://microrna.sanger.ac.uk). .. The hybridized chips were washed and processed for scanning using a LuxScan 10K/A dual-channel laser Microarray Scanner (CapitalBio Co.).

    Article Title: DNA Methylation-Mediated Repression of miR-886-3p Predicts Poor Outcome of Human Small Cell Lung Cancer
    Article Snippet: Small cell lung cancer (SCLC) is one of the most aggressive types of cancer, yet the pathologic mechanisms underlying its devastating clinical outcome remain elusive.. In this report, we surveyed 924 miRNA (miR) for their expressions in the formalin-fixed paraffin-embedded specimens from 42 patients with SCLC, and found that the downregulated miR-886-3p is closely correlated with the shorter survival of SCLC.. This correlation was validated with another 40 cases.

    Article Title: Role of miR-143 targeting KRAS in colorectal tumorigenesis.
    Article Snippet: Briefly, 50mg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction. .. Purified RNA was labeled with Cy3 and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer
    Article Snippet: .. RNA labelling and hybridization on miRNA microarray chips were performed as previously described , by CapitalBio (Capital-Bio Corp., Beijing, China). .. Briefly, 100 μg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction.

    Microarray:

    Article Title: Mouse miRNA-709 directly regulates miRNA-15a/16-1 biogenesis at the posttranscriptional level in the nucleus: evidence for a microRNA hierarchy system
    Article Snippet: Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. Purified RNA was labeled with fluorescein, and hybridization was performed on a miRNA microarray chip (miRNA microarray V4.0, CapitalBio Corp., Beijing, China) containing 1 320 probes in triplicate corresponding to 988 human, 627 mouse and 350 miRNA genes. .. RNA isolation and qRT-PCR of mature miRNAs Total RNA was extracted from cells or tissues using Trizol reagent (Invitrogen). qRT-PCR was carried out using TaqMan miRNA probes (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

    Article Title: Identification of miRNAs that are associated with tumor metastasis in neuroblastoma.
    Article Snippet: 39 .6 9] a t 0 4: 12 3 0 A ug us t 2 01 5 450 Cancer Biology & Therapy Volume 9 Issue 6 previously described.35,36 Briefly, 50 μg total RNA was purified using mirVANA miRNA isolation kit (Ambion, Inc.) to enrich small RNA fraction. .. Purified RNA was labeled with fluorescein and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human and mouse miRNA genes. ..

    Article Title: δ-Opioid Receptor Activation Modified MicroRNA Expression in the Rat Kidney under Prolonged Hypoxia
    Article Snippet: In brief, 50 μg of total RNA were purified using the mirVANA miRNA isolation kit (Ambion, Carlsbad, CA, USA) to enrich for the small RNA fraction. .. The purified RNA was labeled with Cy3 and hybridization was carried out on a miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: Ethanol exposure induces differential microRNA and target gene expression and teratogenic effects which can be suppressed by folic acid supplementation.
    Article Snippet: Briefly, total RNA was extracted from E17.5 fetus brains using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. .. An aliquot (5 mg) of the RNA sample was used for hybridization on miRNA microarray chips (CapitalBio Co., Beijing, China) which contained 509 mature miRNA probes spotted in quadruplicate (435 for human, 261 for mouse and 196 for rat) with annotated active sites, according to the miRNA database at Sanger Center in October 2005 (http://microrna.sanger.ac.uk). .. The hybridized chips were washed and processed for scanning using a LuxScan 10K/A dual-channel laser Microarray Scanner (CapitalBio Co.).

    Article Title: DNA Methylation-Mediated Repression of miR-886-3p Predicts Poor Outcome of Human Small Cell Lung Cancer
    Article Snippet: Small cell lung cancer (SCLC) is one of the most aggressive types of cancer, yet the pathologic mechanisms underlying its devastating clinical outcome remain elusive.. In this report, we surveyed 924 miRNA (miR) for their expressions in the formalin-fixed paraffin-embedded specimens from 42 patients with SCLC, and found that the downregulated miR-886-3p is closely correlated with the shorter survival of SCLC.. This correlation was validated with another 40 cases.

    Article Title: Role of miR-143 targeting KRAS in colorectal tumorigenesis.
    Article Snippet: Briefly, 50mg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction. .. Purified RNA was labeled with Cy3 and hybridization was carried out on miRNA microarray chip (CapitalBio Corp., Beijing, China) containing 509 probes in triplicate, corresponding to 435 human miRNA genes. ..

    Article Title: MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer
    Article Snippet: .. RNA labelling and hybridization on miRNA microarray chips were performed as previously described , by CapitalBio (Capital-Bio Corp., Beijing, China). .. Briefly, 100 μg total RNA was purified by using mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) to enrich small RNA fraction.

    other:

    Article Title: MicroRNA-dependent regulation of PTEN after arsenic trioxide treatment in bladder cancer cell line T24.
    Article Snippet: Arsenic trioxide has shown remarkable biological activity against bladder cancer in some clinical studies.. However, the mechanism of its action is unknown.. Our aim was to find the relationship between miRNAs and arsenic trioxide treatment by using T24 human bladder carcinoma cells.

    Sequencing:

    Article Title: DNA Methylation-Mediated Repression of miR-886-3p Predicts Poor Outcome of Human Small Cell Lung Cancer
    Article Snippet: Small cell lung cancer (SCLC) is one of the most aggressive types of cancer, yet the pathologic mechanisms underlying its devastating clinical outcome remain elusive.. In this report, we surveyed 924 miRNA (miR) for their expressions in the formalin-fixed paraffin-embedded specimens from 42 patients with SCLC, and found that the downregulated miR-886-3p is closely correlated with the shorter survival of SCLC.. This correlation was validated with another 40 cases.



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